Journal: Experimental and Therapeutic Medicine

Article Title: Inhibition of antiviral drug cidofovir on proliferation of human papillomavirus-infected cervical cancer cells

doi: 10.3892/etm.2016.3718

Figure Lengend Snippet: (A) Western blots and (B) quantified western blotting results, showing the expression levels of E6 and p53 proteins treated with CDV and DDP. Values are expressed as the mean ± standard deviation for three wells in each group. *P<0.05 vs. control group. CDV, cidofovir; DDP, cisplatin.

Article Snippet: In addition, anti-mouse FITC secondary antibody (200-032-037) was purchased from Jackson Immunoresearch Inc., (West Grove, PA, USA), while the p53 anti-mouse (BA0521) and anti-mouse β-actin (BA2305) antibodies were purchased from Boster Biological Technology, Ltd., (Wuhan, China).

Techniques: Western Blot, Expressing, Standard Deviation, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Inhibition of antiviral drug cidofovir on proliferation of human papillomavirus-infected cervical cancer cells

doi: 10.3892/etm.2016.3718

Figure Lengend Snippet: Expression levels of E6 and p53 proteins treated with DDP and CDV.

Article Snippet: In addition, anti-mouse FITC secondary antibody (200-032-037) was purchased from Jackson Immunoresearch Inc., (West Grove, PA, USA), while the p53 anti-mouse (BA0521) and anti-mouse β-actin (BA2305) antibodies were purchased from Boster Biological Technology, Ltd., (Wuhan, China).

Techniques: Expressing, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Inhibition of antiviral drug cidofovir on proliferation of human papillomavirus-infected cervical cancer cells

doi: 10.3892/etm.2016.3718

Figure Lengend Snippet: Immunofluorescence microscopy detection of p53 expression in HeLa cells treated by CDV and DDP (magnification, ×1,000). Nuclei were shown in the DAPI-stained cell (blue) and p53 protein was shown as green fluorescence. CDV, cidofovir; DDP, cisplatin.

Article Snippet: In addition, anti-mouse FITC secondary antibody (200-032-037) was purchased from Jackson Immunoresearch Inc., (West Grove, PA, USA), while the p53 anti-mouse (BA0521) and anti-mouse β-actin (BA2305) antibodies were purchased from Boster Biological Technology, Ltd., (Wuhan, China).

Techniques: Immunofluorescence, Microscopy, Expressing, Staining, Fluorescence

Journal: Scientific Reports

Article Title: Rhoifolin from Plumula Nelumbinis exhibits anti-cancer effects in pancreatic cancer via AKT/JNK signaling pathways

doi: 10.1038/s41598-022-09581-3

Figure Lengend Snippet: Bioinformatics analysis of DEPs. All 522 DEPs underwent the analysis of subcellular localization ( A ), the enrichment analysis of KEGG signaling pathways ( B ) and Gene Ontology annotation ( C ). ( D ) The DEPs in the “Proteoglycans in cancer” KEGG pathway. ( E ) Western blot analysis of TGF-β2, SMAD2, and p-SMAD2 in PANC-1 cells treated with or without rhoifolin. GAPDH was used as internal reference.

Article Snippet: After being blocked for 1 h at room temperature, the membrane was incubated with antibodies to AKT (1:2000, 2920, CST), p-AKT (1:2000, 4060, CST), JNK (1:1000, 9258, CST), p-JNK (1:1000, 4668, CST), cleaved caspase3/caspase3 (1:1000, 9662, CST), caspase12 (1:1000, 55238-1-AP, Proteintech), TGF-β2 (1:1000, BA0526-2, Boster), SMAD2 (1:1000, 5339, CST), p-SMAD2 (1:1000, 18338, CST), and GAPDH (1:8000, 60004-1-lg, Proteintech) overnight.

Techniques: Western Blot

Journal: Frontiers in Cellular Neuroscience

Article Title: Adapentpronitrile, a New Dipeptidyl Peptidase-IV Inhibitor, Ameliorates Diabetic Neuronal Injury Through Inhibiting Mitochondria-Related Oxidative Stress and Apoptosis

doi: 10.3389/fncel.2018.00214

Figure Lengend Snippet: List of primary and secondary antibodies used in the study.

Article Snippet: Membranes were probed overnight at 4°C with primary antibodies for specific detection of APP (dilution 1:500; Boster, China, BA0581), Aβ (dilution 1:1000; Abcam, United Kingdom, ab62658), GLP-1R (dilution 1:500, Bioss, China, bs-1559R), Bcl-2 (dilution 1:500; Abcam, United Kingdom, ab196495), Bax (dilution 1:400; Proteintech, China, 50599-2-Ig), cytochrome c (dilution 1:1000; Abcam, United Kingdom, ab133504), caspase-9 (dilution 1:1000; Abcam, United Kingdom, ab184786), caspase-3 (dilution 1:1000; Abcam, United Kingdom, ab184787), and β-actin (dilution 1:3000; Proteintech, United States, 60008-1-Ig; Table ).

Techniques:

Journal: Frontiers in Cellular Neuroscience

Article Title: Adapentpronitrile, a New Dipeptidyl Peptidase-IV Inhibitor, Ameliorates Diabetic Neuronal Injury Through Inhibiting Mitochondria-Related Oxidative Stress and Apoptosis

doi: 10.3389/fncel.2018.00214

Figure Lengend Snippet: Effects of adapentpronitrile on pathomorphology and the expression of APP and Aβ proteins in hippocampus and cortex of rat. (A) Representative neuronal pathomorphology in hippocampus and cortex. Sections were pictured at 400×. Scale bar = 50 μm. (B) The expressions of APP and Aβ proteins were measured by Western Blotting. Data were expressed as mean ± SD of four independent experiments, and were analyzed statistically using one-way ANOVA, followed by Dunnett-t type multiple comparison tests. # P < 0.05 and ## P < 0.01 vs. Control group, respectively; ∗ P < 0.05 and ∗∗ P < 0.01 vs. HFD/STZ group, respectively.

Article Snippet: Membranes were probed overnight at 4°C with primary antibodies for specific detection of APP (dilution 1:500; Boster, China, BA0581), Aβ (dilution 1:1000; Abcam, United Kingdom, ab62658), GLP-1R (dilution 1:500, Bioss, China, bs-1559R), Bcl-2 (dilution 1:500; Abcam, United Kingdom, ab196495), Bax (dilution 1:400; Proteintech, China, 50599-2-Ig), cytochrome c (dilution 1:1000; Abcam, United Kingdom, ab133504), caspase-9 (dilution 1:1000; Abcam, United Kingdom, ab184786), caspase-3 (dilution 1:1000; Abcam, United Kingdom, ab184787), and β-actin (dilution 1:3000; Proteintech, United States, 60008-1-Ig; Table ).

Techniques: Expressing, Western Blot, Comparison, Control